goat anti chordin  (R&D Systems)

Journal: REPRODUCTION

Article Title: Granulosal and thecal expression of bone morphogenetic protein- and activin-binding protein mRNA transcripts during bovine follicle development and factors modulating their expression in vitro

doi: 10.1530/rep-11-0150

Figure Lengend Snippet: Figure 3 Immunohistochemical staining of bovine ovary sections showing the distribution of chordin, gremlin, noggin and follistatin immunoreactivity (brown) in the granulosa and theca layer of small antral follicles (w3 to 5 mm diameter). In eachpanel the left-hand image shows an antibody-treated section while the right-hand image shows an adjacent section treated with the appropriate non-immune serum or purified IgG. The scale bar in each right panel represents 50 mm.

Article Snippet: The following primary antibodies (and appropriately matched control serum/purified IgG for adjacent tissue section) were diluted in blocking buffer and incubated with sections overnight at 4 8C: goat anti-mouse gremlin (R&D Systems) at 5 mg/ml, goat anti-mouse chordin (R&D Systems) at 5 mg/ml, rabbit anti-human noggin (Abcam Ltd, Cambridge, UK) at 5 mg/ml and rabbit anti-human follistatin (FP22; supplied by NIDDK, Baltimore, MD, USA) at 1:500 dilution.

Techniques: Immunohistochemical staining, Staining